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KMID : 0365819910310020143
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Journal of Pusan Medical College
1991 Volume.31 No. 2 p.143 ~ p.155
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The Effect of Catecholarmines and EDTA on the Na-K-ATPase Activity in Rat Cerebral Cortex
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Abstract
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The effect of catecholamines, EDTA and divalent metal ions on the Na-K-ATPase activity in rat cerebral homogenate have been investigated.
The results were as follows
1. Noradrenaline, dopamine, isoproterenol and adrenaline stimulated the Na-K-ATPase activity in dosedependent manner.
2. EDTA could mimic the effects of catecholamines. and EDTA increased the Na-K-ATPase activity in dose-dependent manner.
3. Catecholamines increased the Na-K-ATPase activity at an EDTA concentration of 10´ M whereas in the absence of EDTA the increase of Na-K-ATPase activity produced by catecholamines decreased. Little increase of Na-K-ATPase activity produced by catecholamine occurred at an EDTA concentration of 10-3 M.
4. Ca?´, Zn2+ and Fe"´ inhibited the Na-K-ATPase activity. Dopamine at 10´ M and noradrenaline at 10-4 M were completely effective in reversing the inhibitory effect of Fe"´ but ineffective in reversing the inhibitory effect of Cat+ and Zn".
5. Effects of noradrenaline on Fe" inhibition of Na-K-ATPase activity were only partial in reversing the inhibition of enzyme activity by phentolamine and propranolol.
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